IMMUNOELECTROBLOTTINGS Meaning and
Definition
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Immunoelectroblotting is a laboratory technique used in molecular biology and immunology that combines the principles of western blotting and immunohistochemistry to detect and analyze specific proteins. It is a powerful method that allows researchers to determine the presence and quantity of specific proteins in a sample.
The process of immunoelectroblotting involves several steps. First, proteins are separated by size using gel electrophoresis, typically using polyacrylamide gels. After electrophoresis, the proteins are transferred from the gel to a solid support such as a nitrocellulose or PVDF membrane using an electrical current. This step is known as blotting.
Once the proteins are transferred to the membrane, immunoelectroblotting involves the detection of specific proteins using antibodies. The membrane is incubated with primary antibodies that specifically bind to the protein of interest. These antibodies can be polyclonal or monoclonal and are typically raised against a specific protein or epitope.
Following primary antibody incubation, the membrane is washed to remove any unbound antibodies, and then incubated with secondary antibodies that are conjugated to enzymes or fluorescent markers. These secondary antibodies recognize and bind to the primary antibodies, amplifying the signal.
Finally, the presence of the targeted protein is visualized using various detection methods, such as chemiluminescence or fluorescence, depending on the conjugated marker. The intensity of the signal is proportional to the amount of protein present in the initial sample.
Immunoelectroblotting has widespread applications in research, including the study of protein expression, protein-protein interactions, and disease biomarkers.
Common Misspellings for IMMUNOELECTROBLOTTINGS
- ummunoelectroblottings
- jmmunoelectroblottings
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- 9mmunoelectroblottings
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- immhnoelectroblottings
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- immuboelectroblottings
- immumoelectroblottings
Etymology of IMMUNOELECTROBLOTTINGS
The word "Immunoelectroblotting" is a compound word consisting of three parts: "Immuno-", "electro-", and "blotting".
1. "Immuno-" refers to "immunology", which is the branch of science that studies the immune system and its functioning, including the body's defense against diseases and foreign substances. The term "immuno-" is derived from the Latin word "immunis", meaning "exempt" or "free from".
2. "Electro-" is a prefix derived from the Greek word "electron", which means "amber". It is often used to indicate the involvement of electricity or electric charge in a process.
3. "Blotting" refers to a laboratory technique called "western blotting". It involves the transfer of proteins from a gel to a membrane for further analysis and detection using specific antibodies.
