IMMUNOELECTROOSMOPHORESIS Meaning and
Definition
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Immunoelectroosmophoresis, also known as electroimmunophoresis, is a technique used in immunology and biochemistry to separate and analyze proteins based on their electrical charge and size using an applied electric field. This method combines the principles of immunodiffusion and electrophoresis to detect and characterize antigens and antibodies.
In immunoelectroosmophoresis, a sample containing proteins is applied to a gel medium, typically agarose or polyacrylamide, which is then placed between two electrodes. An electric field is applied, causing the proteins to move through the gel. As the proteins migrate, they separate based on their charge and size, forming distinct bands or precipitin lines.
To specifically detect and analyze antigens or antibodies, immunoelectroosmophoresis utilizes the specific interaction between an antigen and its corresponding antibody. Immunoprecipitation occurs when the antigen and antibody bind, forming a large immune complex that remains immobile in the gel due to size and charge. This precipitin line can be visualized and quantified for further analysis.
Immunoelectroosmophoresis is a versatile technique that offers several advantages, including its ability to detect multiple proteins simultaneously and its high sensitivity. It is commonly employed in clinical laboratories to diagnose and monitor immune-related disorders, such as autoimmune diseases and infections, by identifying specific antigens or detecting the presence of antibodies. Additionally, it aids in the production of antibodies and vaccines, as it allows for the purification and characterization of desired proteins.
Common Misspellings for IMMUNOELECTROOSMOPHORESIS
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Etymology of IMMUNOELECTROOSMOPHORESIS
The word "Immunoelectroosmophoresis" is a combination of several terms:
1. Immuno-: derived from the Latin word "immunis" meaning "exempt" or "free from", it refers to the immune system or immunity.
2. Electro-: related to electricity or electric currents.
3. Osmo-: derived from the Greek word "osmos" meaning "a push or thrust", it refers to the movement of fluid through a semipermeable membrane.
4. -phoresis: derived from the Greek word "phoresis" meaning "carrying", it refers to the movement of charged particles in an electric field.
