ENDONUCLEASE ECORI Meaning and
Definition
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Endonuclease EcoRI is a specific type II restriction enzyme derived from the bacterium Escherichia coli strain R. It is commonly used in molecular biology and genetic engineering applications to manipulate DNA molecules. This enzyme recognizes a specific DNA sequence known as a recognition site or restriction site, and cleaves the DNA at the site of recognition.
Endonuclease EcoRI belongs to the EcoRI restriction-modification system, which acts as the bacterial defense mechanism against invading foreign DNA. It protects the bacterium by recognizing and cutting DNA molecules that contain non-methylated recognition sites, which are often found in viral genomes or plasmids.
The recognition site of EcoRI consists of six base pairs: 5'-GAATTC-3'. EcoRI cleaves the DNA strand between the neighboring adenine (A) and the first thymine (T) in this sequence, generating characteristic 'sticky ends.' These sticky ends have unpaired nucleotides at their ends and allow for subsequent DNA molecules to be easily joined together through complementary base pairing.
Endonuclease EcoRI exhibits high specificity and efficiency, and its recognition site is relatively common in DNA sequences, occurring approximately once every 256 base pairs in average DNA. This attribute makes EcoRI one of the most frequently used restriction enzymes in genetic engineering experiments. It is an essential tool for DNA cloning, DNA fingerprinting, and other molecular biology techniques.
Common Misspellings for ENDONUCLEASE ECORI
- wndonuclease ecori
- sndonuclease ecori
- dndonuclease ecori
- rndonuclease ecori
- 4ndonuclease ecori
- 3ndonuclease ecori
- ebdonuclease ecori
- emdonuclease ecori
- ejdonuclease ecori
- ehdonuclease ecori
- ensonuclease ecori
- enxonuclease ecori
- enconuclease ecori
- enfonuclease ecori
- enronuclease ecori
- eneonuclease ecori
- endinuclease ecori
- endknuclease ecori
- endlnuclease ecori
- endpnuclease ecori
Etymology of ENDONUCLEASE ECORI
The word "endonuclease EcoRI" has a specific etymology related to its scientific nomenclature.
"Endonuclease" comes from a combination of two Greek roots: "endon" meaning "within" or "inside" and "nuclease" which refers to an enzyme that breaks down nucleic acids like DNA or RNA. So "endonuclease" can be understood as an enzyme that cleaves or cuts nucleic acids from within.
"EcoRI" on the other hand, is derived from Escherichia coli (E. coli) which is a bacterium commonly used in scientific research and named after Theodor Escherich, who first isolated the strain. The letter "R" in EcoRI signifies the order in which restriction enzymes from E. coli were discovered.
Therefore, "Endonuclease EcoRI" denotes a specific type of endonuclease enzyme derived from the E.
