ENDONUCLEASE BSTFI Meaning and
Definition
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Endonuclease BstFI is a type II restriction enzyme that belongs to the family of restriction enzymes known as BstFI-like enzymes. These enzymes are classified as type II because they require only a single protein for their activity and function by recognizing specific DNA sequences and cleaving the DNA within or near those sequences.
BstFI is derived from the bacterium Bacillus stearothermophilus, and its name is derived from the acronym of the bacterial species. It recognizes and binds to the DNA sequence 5'-CGCG-3', or its reverse complement 5'-GCGC-3', and cleaves the DNA at specific points within this recognition site. This cleavage results in the production of double-stranded DNA fragments with an overhang at the cleavage site. The overhangs generated by BstFI are four bases long, with two bases on each DNA strand, thus creating a 4-base pair sticky end.
Endonuclease BstFI is widely used in molecular biology and genetic research for its ability to specifically cleave DNA at these target sites. It has applications in techniques such as restriction fragment length polymorphism (RFLP) analysis, site-directed mutagenesis, cloning, and DNA sequencing. The enzyme is commercially available and can be used in conjunction with other enzymes, such as DNA ligase, to manipulate DNA molecules and create specific DNA constructs for various purposes in biotechnology and genetic engineering.
Common Misspellings for ENDONUCLEASE BSTFI
- wndonuclease bstfi
- sndonuclease bstfi
- dndonuclease bstfi
- rndonuclease bstfi
- 4ndonuclease bstfi
- 3ndonuclease bstfi
- ebdonuclease bstfi
- emdonuclease bstfi
- ejdonuclease bstfi
- ehdonuclease bstfi
- ensonuclease bstfi
- enxonuclease bstfi
- enconuclease bstfi
- enfonuclease bstfi
- enronuclease bstfi
- eneonuclease bstfi
- endinuclease bstfi
- endknuclease bstfi
- endlnuclease bstfi
- endpnuclease bstfi
Etymology of ENDONUCLEASE BSTFI
The word "Endonuclease BstFI" is derived from multiple sources:
1. Endonuclease: The term "endonuclease" comes from the combination of two Greek roots - "endo" meaning "within" and "nuclease" which refers to an enzyme that breaks down nucleic acids. So, "endonuclease" literally means an enzyme that cleaves nucleic acids internally.
2. BstFI: "BstFI" is derived from the name of the bacterium Bacillus stearothermophilus from which the enzyme was initially isolated. The abbreviation "Bst" stands for Bacillus stearothermophilus and "FI" refers to the order in which the enzyme was discovered.
Therefore, "Endonuclease BstFI" refers to an enzyme that cleaves nucleic acids internally and is isolated from the bacterium Bacillus stearothermophilus.
