ENDONUCLEASE CHUI Meaning and
Definition
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Endonuclease ChuI is a type II restriction enzyme that is commonly used in molecular biology research for various DNA manipulation techniques. It is derived from the bacterium Chlamydia hamosadovikii, which is found in the digestive tracts of birds.
Endonucleases are enzymes that can cleave the phosphodiester bonds within the DNA strand, resulting in the separation of the DNA molecule into smaller fragments. ChuI specifically recognizes and cuts DNA at a specific nucleotide sequence known as the recognition site or recognition sequence. The recognition sequence for ChuI is 5'-GCT↓CTTC-3', where the arrow indicates the cleavage site.
Endonuclease ChuI is classified as a type II restriction endonuclease because it cleaves DNA at a specific site within its recognition sequence, as opposed to type I enzymes that cleave DNA randomly. This makes ChuI particularly useful for DNA digestion and analysis in the laboratory.
The cleavage activity of Endonuclease ChuI results in the formation of DNA fragments with sticky ends, meaning the cuts are not blunt but leave overhanging nucleotides. These sticky ends can then be used to ligate or join DNA fragments with complementary overhangs, a crucial step in molecular cloning techniques.
Overall, Endonuclease ChuI is an essential tool in recombinant DNA technology, allowing scientists to manipulate and analyze DNA sequences for various research purposes, including gene cloning, gene expression studies, and DNA sequencing.
Common Misspellings for ENDONUCLEASE CHUI
- wndonuclease chui
- sndonuclease chui
- dndonuclease chui
- rndonuclease chui
- 4ndonuclease chui
- 3ndonuclease chui
- ebdonuclease chui
- emdonuclease chui
- ejdonuclease chui
- ehdonuclease chui
- ensonuclease chui
- enxonuclease chui
- enconuclease chui
- enfonuclease chui
- enronuclease chui
- eneonuclease chui
- endinuclease chui
- endknuclease chui
- endlnuclease chui
- endpnuclease chui
Etymology of ENDONUCLEASE CHUI
The word "endonuclease ChuI" is a combination of two parts: "endonuclease" and "ChuI".
1. "Endonuclease" is derived from the Greek roots "endo-" (meaning "within") and "-nuclease" (related to breaking down), ultimately referring to an enzyme that catalyzes the cleavage of nucleic acids (DNA or RNA) at specific sites within the molecule. The term "endonuclease" was first used in scientific literature in the early 20th century.
2. "ChuI" refers to a specific type of endonuclease named after the bacterium Chlamydia hofmannii strain IAR-351. The name "ChuI" represents the strain or isolate of the bacterium from which this endonuclease was originally discovered or isolated.
