Endonuclease GdoI, also known as GdoI endonuclease, is an enzyme that belongs to the family of restriction enzymes. It is derived from the bacteria Geobacillus stearothermophilus and is commonly used in molecular biology and genetic engineering techniques.
Endonucleases are enzymes that cleave DNA or RNA molecules at specific sites within the nucleotide sequence. They play a vital role in genetic recombination, repairing damaged DNA, and in gene expression regulation. Endonuclease GdoI is an example of a type II restriction endonuclease, which means it recognizes a specific DNA sequence and cleaves it at a defined position internally.
GdoI recognizes a palindromic DNA sequence, meaning that it reads the same forward and backward. The specific recognition sequence for GdoI is usually 5'-GCC-GGC-3'. Once the GdoI endonuclease binds to its recognition sequence, it cleaves the DNA strand on both strands at defined positions. This results in the generation of specific DNA fragments with sticky ends, which are compatible with other DNA fragments cleaved by this enzyme.
Endonuclease GdoI is widely used in molecular biology research, particularly in DNA cloning and gene editing. It enables the manipulation of DNA molecules by selectively cutting them at specific locations, facilitating the insertion of foreign DNA fragments into DNA vectors or the study of gene function through targeted mutagenesis.
In summary, Endonuclease GdoI is a restriction enzyme that recognizes and cleaves DNA at specific palindromic sequences, providing valuable tools for DNA manipulation and research purposes.
