ENDONUCLEASE BPEI Meaning and
Definition
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Endonuclease BpeI is a type II restriction endonuclease enzyme that is derived from the bacterium Burkholderia pertussis. It belongs to the BamHI restriction-modification system and is commonly used in molecular biology research and genetic engineering applications.
Endonucleases are a class of enzymes that cleave DNA or RNA molecules by breaking the phosphodiester bonds within the nucleic acid chain. Specifically, BpeI acts as an endonuclease enzyme, meaning it cleaves DNA molecules at specific recognition sites found within the DNA sequence.
The BpeI endonuclease recognizes a specific DNA sequence, 5'–GATC–3', and cuts the DNA at this site. This recognition sequence is called a restriction site and is usually palindromic, meaning it reads the same on both DNA strands when oriented in the 5' to 3' direction.
The cleavage of DNA by BpeI endonuclease generates double-stranded DNA fragments with blunt ends, as it cuts both DNA strands at the same position. These DNA fragments can then be used in various molecular biology techniques such as DNA cloning, DNA sequencing, and gene expression studies.
To ensure proper DNA cleavage, BpeI endonuclease requires certain cofactors or coenzymes, such as magnesium ions (Mg2+), for its enzymatic activity. It is commonly used alongside other restriction enzymes and DNA modifying enzymes to manipulate DNA fragments for various laboratory applications.
Common Misspellings for ENDONUCLEASE BPEI
- wndonuclease bpei
- sndonuclease bpei
- dndonuclease bpei
- rndonuclease bpei
- 4ndonuclease bpei
- 3ndonuclease bpei
- ebdonuclease bpei
- emdonuclease bpei
- ejdonuclease bpei
- ehdonuclease bpei
- ensonuclease bpei
- enxonuclease bpei
- enconuclease bpei
- enfonuclease bpei
- enronuclease bpei
- eneonuclease bpei
- endinuclease bpei
- endknuclease bpei
- endlnuclease bpei
- endpnuclease bpei
Etymology of ENDONUCLEASE BPEI
The term "endonuclease BpeI" is a compound term consisting of two main components: "endonuclease" and "BpeI".
"Endonuclease" is derived from two Greek roots: "endo", meaning "within", and "nuclease", which comes from the Latin word "nucleus", referring to the cell nucleus. Therefore, "endonuclease" literally means "within the nucleus". In biology and biochemistry, an endonuclease is an enzyme that cleaves DNA or RNA at specific internal sites.
"BpeI" is a specific name given to the endonuclease. The name "BpeI" typically consists of three parts: a capital letter indicating the genus, a lowercase letter indicating the species, and a Roman numeral indicating the order of discovery.
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