Peroxidase Labeled Antibody Techniques refer to a laboratory methodology frequently used in immunohistochemistry and immunoassay techniques to detect the presence of specific antigens or proteins in a biological sample.
This technique involves the use of specific antibodies conjugated or labeled with peroxidase enzyme. Peroxidase is an enzyme that catalyzes chemical reactions by facilitating the transfer of oxygen atoms from hydrogen peroxide to various substrates, resulting in the generation of colored dyes or fluorescent molecules as end products.
In this technique, the peroxidase-labeled antibodies bind to the target antigen of interest present in the sample. Then, hydrogen peroxide and a chromogenic substrate such as 3,3'-diaminobenzidine (DAB), which can be oxidized by peroxidase, are added. The peroxidase enzyme catalyzes the reaction between hydrogen peroxide and the chromogenic substrate, resulting in the formation of a colored insoluble product. This colored product can be visualized under a microscope or quantitatively measured using spectrophotometric techniques.
Peroxidase labeled antibody techniques are extensively used due to their high sensitivity, specificity, and versatility. They offer a wide range of applications in various fields including research, diagnostics, and pathology. These techniques are particularly valuable for detecting and localizing proteins, antigens, or other molecules of interest in tissues, cells, or other biological samples, contributing to the understanding of their distribution, function, and association with diseases or conditions.
