Peroxidase Labeled Antibody technique, also known as the peroxidase-labeled antibody method or peroxidase-labeled immunoassay, is a laboratory technique widely used in various research fields, particularly in immunology and medical diagnostics.
In this technique, an enzyme called peroxidase is coupled or labeled to an antibody molecule. Antibodies are proteins produced by the immune system in response to the presence of foreign substances known as antigens. These antigens can be specific molecules or parts of microorganisms that trigger an immune response.
The peroxidase-labeled antibody technique allows the detection and quantification of antigens or antibodies in a sample. When the peroxidase-labeled antibody reacts with its specific antigen in the sample, the enzyme catalyzes a reaction that produces a measurable signal. This can be achieved by adding a colorimetric substrate that changes color in the presence of peroxidase, which allows visual detection, or by using a spectrophotometer to measure the change in absorbance.
This technique is highly sensitive and specific and is widely used in applications such as enzyme-linked immunosorbent assays (ELISAs), immunohistochemistry, flow cytometry, and Western blotting. It enables researchers and healthcare professionals to detect and quantify various biomarkers, such as hormone levels, viral or bacterial antigens, cancer markers, and antibodies specific to certain diseases.
In summary, the peroxidase-labeled antibody technique is a powerful tool in immunological research and clinical diagnostics, allowing the detection and quantification of specific antigens or antibodies in biological samples using peroxidase-labeled antibodies.
