IMMUNOPRECIPITATION Meaning and
Definition
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Immunoprecipitation is a scientific technique that involves the selective isolation and purification of a specific protein or group of proteins from a complex mixture using an antibody. It is often used in molecular biology and biochemistry research to study protein-protein interactions, protein localization, and protein expression levels.
The process of immunoprecipitation begins by incubating a cell lysate or tissue extract containing the target protein(s) with a specific antibody that recognizes and binds to the protein(s) of interest. This forms an immune complex, in which the antibody is bound to the target protein(s). To separate the immune complex from other proteins and cellular components, an additional step is needed. One common method is the addition of protein A or protein G coated agarose beads, which have a high affinity for the constant region of antibodies. These beads bind to the immune complex, allowing for the subsequent separation of the immune complex through centrifugation or filtration.
Once the immune complex is isolated, it can be further analyzed through various techniques such as Western blotting, mass spectrometry, or sequencing to gain insights into the properties and functions of the target protein(s). Immunoprecipitation provides a versatile and powerful tool for studying protein-protein interactions and allows for the identification and characterization of proteins in complex mixtures.
Common Misspellings for IMMUNOPRECIPITATION
- ummunoprecipitation
- jmmunoprecipitation
- kmmunoprecipitation
- ommunoprecipitation
- 9mmunoprecipitation
- 8mmunoprecipitation
- inmunoprecipitation
- ikmunoprecipitation
- ijmunoprecipitation
- imnunoprecipitation
- imkunoprecipitation
- imjunoprecipitation
- immynoprecipitation
- immhnoprecipitation
- immjnoprecipitation
- imminoprecipitation
- imm8noprecipitation
- imm7noprecipitation
- immuboprecipitation
- immumoprecipitation
Etymology of IMMUNOPRECIPITATION
The word "Immunoprecipitation" is derived from multiple roots:
1. "Immunos" - From the Latin word "immunis", meaning "free from obligation, exempt". In the context of immunology, it refers to the immune system's ability to recognize and defend against foreign substances.
2. "Precipitation" - From the Latin word "praecipitare", which means "to throw down" or "to hasten". In scientific terms, precipitation refers to the process of causing a dissolved substance to separate from a solution.
3. "Ion" - From the Greek word "ion", meaning "going". In chemistry, an ion refers to a charged particle formed by the loss or gain of electrons.
