HEMOLYTIC TITRATION ASSAY Meaning and
Definition
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A hemolytic titration assay is a laboratory technique used to determine the concentration of antibodies or complement proteins that can cause the destruction of red blood cells, known as hemolysis. This assay is primarily used in immunology and clinical laboratories to assess the immune response and detect the presence and severity of certain diseases or autoimmune disorders.
In a hemolytic titration assay, a known volume of red blood cells is mixed with a series of dilutions of the test sample, which may contain antibodies or complement proteins. The mixture is then incubated, allowing the antibodies or complement proteins to bind to the red blood cells. After incubation, the mixture is centrifuged to separate the intact red blood cells from the lysed ones. The degree of hemolysis is often determined by measuring the color intensity of the supernatant using a spectrophotometer.
By performing a series of dilutions, the assay allows for the quantification of the antibodies or complement proteins present in the test sample. The endpoint of the assay is usually determined by the dilution at which a noticeable decrease in the hemolytic activity is observed.
Hemolytic titration assays provide valuable information about the activity and concentration of antibodies or complement proteins, aiding in the diagnosis and monitoring of conditions such as autoimmune hemolytic anemia, transfusion reactions, and immune deficiencies.
Common Misspellings for HEMOLYTIC TITRATION ASSAY
- gemolytic titration assay
- bemolytic titration assay
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- hemilytic titration assay
- hemklytic titration assay
- hemllytic titration assay
- hemplytic titration assay
- hem0lytic titration assay
