CDNA ARRAYS Meaning and
Definition
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cDNA arrays, also known as DNA microarrays or gene chips, are a powerful tool used in molecular biology and genetics to study gene expression patterns on a large scale. These arrays consist of thousands or even millions of individual DNA spots immobilized on a solid surface, such as a glass slide or silicon chip.
The cDNA in cDNA arrays refers to complementary DNA, which is synthesized from messenger RNA (mRNA) molecules extracted from cells or tissues of interest. Complementary DNA is a single-stranded DNA molecule synthesized using an enzyme called reverse transcriptase, which converts mRNA into cDNA by using a DNA template called a primer.
cDNA arrays enable researchers to analyze the expression of thousands of genes simultaneously. The cDNA spots on the arrays represent specific genes, and by hybridizing the cDNA with fluorescently labeled samples of mRNA from different cell or tissue types, researchers can determine the relative abundance of each mRNA in the samples. The fluorescence emitting from each spot on the array is detected and quantified using specialized scanners and image analysis software.
This high-throughput approach allows researchers to study gene expression patterns in different conditions, such as healthy versus diseased cells or before and after treatment with a particular drug. By identifying genes that are upregulated or downregulated under specific conditions, cDNA arrays can provide insights into the molecular mechanisms underlying various biological processes, diseases, and drug responses.
Overall, cDNA arrays are a valuable tool for large-scale gene expression profiling and have revolutionized the field of genomics.
Common Misspellings for CDNA ARRAYS
- xdna arrays
- vdna arrays
- fdna arrays
- ddna arrays
- csna arrays
- cxna arrays
- ccna arrays
- cfna arrays
- crna arrays
- cena arrays
- cdba arrays
- cdma arrays
- cdja arrays
- cdha arrays
- cdnz arrays
- cdns arrays
- cdnw arrays
- cdnq arrays
- cdna zrrays
- cdna srrays
Etymology of CDNA ARRAYS
The etymology of the term "cDNA arrays" can be broken down as follows:
1. cDNA: This stands for "complementary DNA". Complementary DNA is synthesized from messenger RNA (mRNA) through a process called reverse transcription. It involves using an enzyme called reverse transcriptase to generate a DNA copy of the original RNA molecule. The "c" in cDNA denotes that it is complementary to the original RNA sequence.
2. Arrays: In the context of cDNA arrays, "arrays" refers to a collection or arrangement of genetic material on a solid support, such as a glass slide or silicon chip. In this technology, cDNA samples derived from different RNA sources are spotted onto the solid support in a spatially defined pattern, allowing for simultaneous analysis of gene expression levels for multiple genes.
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