CDNA Meaning and
Definition
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CDNA, also known as complementary DNA, is a type of DNA synthesized from a messenger RNA (mRNA) template through a process called reverse transcription. This molecular biology technique allows for the production of a DNA molecule that is complementary to the sequence of the mRNA molecule.
The process of generating cDNA involves the use of an enzyme called reverse transcriptase, which synthesizes a single-stranded cDNA molecule using the mRNA as a template. This cDNA is then converted into double-stranded DNA using DNA polymerase to improve stability and enable further manipulation.
CDNA is commonly used in molecular biology research to study gene expression and analyze the transcripts present in a particular cell or tissue. By capturing the mRNA sequence and converting it into cDNA, scientists can generate a stable and durable copy of the original genetic material. This cDNA can be amplified through a technique called polymerase chain reaction (PCR) to generate multiple copies for downstream analysis, such as gene cloning, sequencing, or gene expression profiling.
CDNA can provide valuable insights into the gene expression patterns within cells and tissues under various conditions, aiding in the understanding of normal biological processes, disease mechanisms, and drug development. It has contributed significantly to fields like genomics, transcriptomics, and molecular diagnostics, allowing researchers to elucidate the functions and interactions of genes in complex biological systems.
Common Misspellings for CDNA
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