CDNA PROBES Meaning and
Definition
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cDNA probes refer to complementary DNA probes, which are short, single-stranded DNA molecules that are specifically designed to hybridize and bind to complementary sequences of messenger RNA (mRNA) molecules. Complementary DNA (cDNA) is synthesized from mRNA using the enzyme reverse transcriptase, allowing for the production of DNA molecules that mirror the mRNA sequences.
cDNA probes are valuable tools in molecular biology and genetics research as they enable the detection, identification, and isolation of specific mRNA molecules within complex samples. By using cDNA probes, researchers can study the gene expression patterns of cells or tissues, determine the presence and abundance of specific mRNA molecules, and investigate the regulation and function of genes.
Typically, cDNA probes are labeled with radioactive isotopes, fluorescent molecules, or chemiluminescent compounds, allowing for their visualization and detection following hybridization with the target mRNA. Upon hybridization, the cDNA probe forms a stable double-stranded DNA molecule with its complementary mRNA target.
The hybridization process can be performed using techniques like Northern blotting, in situ hybridization, or fluorescence in situ hybridization (FISH). By analyzing the presence and distribution of the hybridized cDNA probes, researchers can gain insights into various biological processes, including development, disease states, and cellular responses.
In conclusion, cDNA probes are short, single-stranded DNA molecules that can selectively bind to complementary mRNA sequences and are used to detect and study the presence, abundance, and distribution of specific mRNA molecules in biological samples.
Common Misspellings for CDNA PROBES
- xdna probes
- vdna probes
- fdna probes
- ddna probes
- csna probes
- cxna probes
- ccna probes
- cfna probes
- crna probes
- cena probes
- cdba probes
- cdma probes
- cdja probes
- cdha probes
- cdnz probes
- cdns probes
- cdnw probes
- cdnq probes
- cdna orobes
- cdna lrobes
Etymology of CDNA PROBES
The term "cDNA probes" is a combination of two separate parts: "cDNA" and "probes".
- cDNA: cDNA is short for complementary DNA. It refers to a synthetic form of DNA that is synthesized from a messenger RNA (mRNA) template through a process called reverse transcription. This technique allows the conversion of mRNA into DNA, which can then be amplified and studied.
- Probes: In molecular biology, a probe is a short piece of DNA or RNA that is used to detect a specific sequence in a larger molecule, typically by binding to it. Probes are commonly used in various techniques like hybridization or PCR (polymerase chain reaction) to identify specific genes or DNA sequences.
The word "probe" itself comes from the Latin word "proba" which means "proof" or "test".
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