How Do You Spell RADIATION HYBRID MAP?

Pronunciation: [ɹˌe͡ɪdɪˈe͡ɪʃən hˈa͡ɪbɹɪd mˈap] (IPA)

The spelling of "Radiation Hybrid Map" is phonetically transcribed as /ˌreɪdiˈeɪʃən ˈhaɪbrɪd mæp/. The word "Radiation" is pronounced as /ˌreɪdiˈeɪʃən/ with stress on the second syllable. "Hybrid" is pronounced as /ˈhaɪbrɪd/ with stress on the first syllable. Lastly, "Map" is pronounced as /mæp/. A radiation hybrid map is a tool used to compare genetic maps of organisms and is constructed by fusing irradiated cells of one species with cells of another species.

RADIATION HYBRID MAP Meaning and Definition

1. A radiation hybrid map is a type of genetic map that is used to determine and position the locations of genes along a chromosome. It is created by using a radiation-induced technique to generate hybrid cell lines containing random breaks and rearrangements in their chromosomes. These hybrid cells are then used to map the positions of genes through a process called radiation hybrid mapping.

   In radiation hybrid mapping, the hybrid cell lines are exposed to ionizing radiation, which induces DNA damage and breaks. The damaged chromosomes from different cell lines are then fused together to create a hybrid cell containing a mixture of genetic material. The frequency at which the breaks and rearrangements occur can vary along the chromosomes, creating a unique set of markers that can be used for mapping.

   To create a radiation hybrid map, the hybrid cells are screened for specific molecular markers, such as microsatellites or DNA sequences, that are known to be located near genes of interest. By analyzing the presence or absence of these markers in the hybrid cells, the relative distances between the markers can be determined. These distances can then be used to estimate the positions of the genes of interest along the chromosome.

   Radiation hybrid mapping has been widely used in genetics and genomics research, especially for mapping genes in species whose genomes have not been fully sequenced. It provides a valuable tool for locating genes associated with specific traits or diseases and is particularly useful for constructing high-resolution maps of the genome.

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