Immunosorbent techniques, also known as immunosorbent assays (ISAs), are laboratory methods used to detect and measure the presence of specific substances, such as antigens or antibodies, in a patient's biological sample. These techniques are based on the principle of antibody-antigen binding and are widely used in various fields of research, diagnostics, and biomedical sciences.
Immunosorbent techniques typically involve the immobilization of an antibody or antigen onto a solid support, such as a microplate or membrane. The sample containing the target substance is then added to the support, allowing the formation of antigen-antibody complexes. The bound complexes are then detected and quantified using various methods, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), or flow cytometry.
Immunosorbent techniques offer high specificity and sensitivity, making them valuable tools for diagnosing diseases, monitoring therapeutic treatments, or detecting the presence of infectious agents. These techniques are widely used in clinical laboratories, biotechnology companies, and research institutions.
Overall, immunosorbent techniques play a crucial role in the field of immunology and provide valuable insights into the functioning of the immune system, the detection of diseases, and the development of novel diagnostic and therapeutic approaches.
