IMMUNOLABELING TECHNIQUE Meaning and
Definition
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Immunolabeling technique refers to a laboratory method used in immunohistochemistry and immunocytochemistry to visualize specific molecules or antigens within cells or tissues. This technique involves the use of specific antibodies that are selectively bound to these molecules, allowing researchers to detect and image their presence and location.
The immunolabeling technique begins with the preparation of the sample, which could be a tissue section or cultured cells. The sample is first fixed to maintain its structure and then permeabilized to allow antibodies to access the target antigens. Next, primary antibodies that are specific to the desired antigen are applied to the sample. These primary antibodies bind specifically to the target molecules.
To visualize the primary antibody-antigen complex, secondary antibodies are applied. These secondary antibodies are labeled with a fluorescent dye or an enzyme that produces a detectable signal. They selectively recognize and bind to the primary antibodies, amplifying the signal and making it visible under a microscope or through other imaging techniques.
In addition to fluorescence and enzyme-based detection, other methods such as immunogold labeling and immunoperoxidase staining can be employed to visualize the target molecules.
Overall, immunolabeling technique is a valuable tool in biomedical research and diagnostics, allowing scientists to study the localization and expression of specific proteins or other molecules in various cellular and tissue contexts.
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Etymology of IMMUNOLABELING TECHNIQUE
The word "immunolabeling technique" is composed of two components: "immuno-" and "labeling".
The prefix "immuno-" derives from the Latin word "immunis", meaning "exempt" or "free from". The term "immunis" originated from "in-" (meaning "not") and "munis" (meaning "service" or "obligation"). In the context of "immunolabeling technique", the prefix "immuno-" relates to the immune system.
The word "labeling" comes from the verb "label", which dates back to the late 14th century and is derived from the Old French word "labele", meaning "ribbon" or "strip". Its origin can be traced to the Latin word "labellum", meaning "a little lip" or "a little flap".
Similar spelling word for IMMUNOLABELING TECHNIQUE
