CELL LYSATE Meaning and
Definition
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Cell lysate refers to a mixture of cellular components that are released or extracted from cells through the process of cell lysis. It is obtained by breaking open cells, causing the release of intracellular contents into a solution.
Cell lysate typically contains a wide range of biomolecules found within a cell, such as proteins, nucleic acids, lipids, and metabolites. The composition of the lysate can vary depending on the type of cell, the method of cell lysis, and the intended application.
To obtain a cell lysate, cells are subjected to physical disruption or chemical methods. Physical methods involve techniques such as sonication, freezing and thawing, or high-pressure homogenization to break apart the cell membrane. Chemical methods can include the use of detergents or enzymes to disrupt the cell membrane and release the cellular contents.
Cell lysates are commonly used in various biological and biochemical experiments. They serve as a valuable source of intracellular proteins and other molecules for studying cellular processes, protein-protein interactions, enzymatic activities, and gene expression. Additionally, lysates are often employed in techniques like Western blotting, enzyme assays, protein purification, and gene expression analysis.
Overall, cell lysate plays a crucial role in molecular and cell biology research, as it enables the investigation of cellular components and their functions in both normal and pathological conditions.
Common Misspellings for CELL LYSATE
- xell lysate
- vell lysate
- fell lysate
- dell lysate
- cwll lysate
- csll lysate
- cdll lysate
- crll lysate
- c4ll lysate
- c3ll lysate
- cekl lysate
- cepl lysate
- ceol lysate
- celk lysate
- celp lysate
- celo lysate
- cell kysate
- cell pysate
- cell oysate
- cell ltsate
Etymology of CELL LYSATE
The word "cell lysate" is a compound noun that consists of two parts: "cell" and "lysate". The etymology of each part is as follows:
1. Cell: The term "cell" comes from the Latin word "cella", which means "small room" or "storeroom". It was first used to describe the basic unit of structure and function in living organisms by the English scientist Robert Hooke in 1665.
2. Lysate: The term "lysate" is derived from the Greek word "lysis", which means "a loosening" or "a dissolution". In the context of biology, "lysis" refers to the breaking down or destruction of cells, often through the disruption of their cell membranes. "Lysate" specifically refers to the resulting substance or solution after cell lysis has occurred.
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