Carbonyl cyanide p-trifluoromethoxyphenylhydrazone (CCCP) is a chemical compound commonly used in research laboratories, particularly in the field of biochemistry. It is an organic compound with the molecular formula C10H5F3N3O, and it belongs to the class of phenylhydrazones.
CCCP is primarily known for its function as an uncoupler of oxidative phosphorylation in cells. It disrupts the electron transport chain in the mitochondria, inhibiting the production of adenosine triphosphate (ATP), which is the main energy currency in cells. As a result, it can cause a decrease in cellular respiration and metabolic activity.
The compound itself is a crystalline solid that is typically white or off-white in color. It is soluble in organic solvents such as ethanol, dimethyl sulfoxide (DMSO), and dimethylformamide (DMF), but it is insoluble in water. CCCP has a characteristic aromatic smell.
Due to its effects on cellular respiration, CCCP is often utilized in various research applications, including studies on mitochondrial function, energy metabolism, and ATP synthesis. It is commonly used as a research tool to investigate the roles of mitochondria in cellular processes and to study the effects of uncoupling agents on cellular physiology.
It is important to note that CCCP is a potent compound and should be handled with caution, as it can be toxic and harmful to living organisms if not handled properly. Proper safety measures should be followed when working with this compound in laboratory settings.
