TMT DISULFIDE Meaning and
Definition
-
TMT Disulfide, also known as Tandem Mass Tag Disulfide, is a chemical compound commonly used in proteomics research. It is a reagent that is employed in mass spectrometry-based techniques for the identification and quantification of proteins.
TMT Disulfide is specifically designed for the labeling of cysteine residues in proteins. Cysteine residues contain sulfhydryl groups, which can form disulfide bonds with other cysteine residues within a protein or with external disulfide-containing molecules. TMT Disulfide takes advantage of these disulfide bond formations to enable protein labeling and subsequent downstream analysis.
In practical use, TMT Disulfide is applied to cysteine residues in a protein sample, usually after proteolytic digestion, to label and differentiate the proteins of interest. Subsequently, the labeled proteins are subjected to mass spectrometry analysis, where they are fragmented and ionized. The resulting mass spectra are then used to identify and quantify the individual proteins based on their distinctive characteristic peptide patterns labeled by TMT Disulfide.
This labeling technique offers several advantages in proteomics research. It enables multiplexing, where multiple protein samples can be labeled with different TMT Disulfide isotopologues, allowing for simultaneous analysis and comparison of protein expression levels. TMT Disulfide is also highly sensitive, providing accurate and reliable quantification results, and it has been widely used for applications such as differential protein expression analysis, protein structural characterization, and protein interaction studies.
Common Misspellings for TMT DISULFIDE
- rmt disulfide
- fmt disulfide
- gmt disulfide
- ymt disulfide
- 6mt disulfide
- 5mt disulfide
- tnt disulfide
- tkt disulfide
- tjt disulfide
- tmr disulfide
- tmf disulfide
- tmg disulfide
- tmy disulfide
- tm6 disulfide
- tm5 disulfide
- tmt sisulfide
- tmt xisulfide
- tmt cisulfide
- tmt fisulfide
- tmt risulfide
Etymology of TMT DISULFIDE
The etymology of the word "TMT Disulfide" can be broken down as follows:
"TMT" stands for the compound Trimethylolpropane Trimethacrylate. "Trimethylolpropane" refers to a polyfunctional compound consisting of three hydroxyl groups and a propane backbone, while "Trimethacrylate" indicates that each of the hydroxyl groups is esterified with a methacrylic acid molecule.
"Disulfide" refers to a type of chemical bond known as a disulfide bond or disulfide bridge, which is formed between two sulfur atoms. This bond is formed when two thiol (-SH) groups react and release a molecule of hydrogen (H2). In the case of "TMT Disulfide", it indicates that there is a disulfide bond present within the compound TMT.
Infographic
Add the infographic to your website:
