SUPERSHIFT Meaning and
Definition
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Supershift is a term commonly used in the context of molecular biology and genetics. It refers to a specific phenomenon that occurs during the analysis of protein-DNA interactions using a technique called gel electrophoresis.
In gel electrophoresis, a mixture of proteins and DNA fragments is separated based on their size and charge. This technique involves the use of an electric field to move the charged molecules through a gel matrix. When a protein interacts with a specific DNA sequence, it forms a stable complex known as a protein-DNA complex.
Supershift occurs when an antibody specific to the protein of interest is added to the protein-DNA mixture before gel electrophoresis. The antibody binds to the protein and creates a larger complex, which migrates more slowly through the gel during electrophoresis. This antibody-induced reduction in mobility on the gel is the supershift.
The supershift is useful in identifying and characterizing specific protein-DNA interactions. By comparing the migration patterns of the protein-DNA complex with and without antibody, researchers can determine whether a protein of interest is involved in the complex formation. This technique provides valuable insights into the binding affinity, specificity, and composition of protein-DNA complexes.
Overall, supershift is a technique used to investigate protein-DNA interactions by introducing an antibody that alters the migration pattern of the protein-DNA complex during gel electrophoresis, aiding in the identification and characterization of these interactions.
Common Misspellings for SUPERSHIFT
- aupershift
- zupershift
- xupershift
- dupershift
- eupershift
- wupershift
- sypershift
- shpershift
- sjpershift
- sipershift
- s8pershift
- s7pershift
- suoershift
- sulershift
- su-ershift
- su0ershift
- supwrshift
- supsrshift
- supdrshift
- suprrshift
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