How Do You Spell SOUTHERN BLOT?

Pronunciation: [sˈʌðən blˈɒt] (IPA)

Southern Blot is a molecular biology technique used to detect a specific DNA sequence in a sample. The term 'Southern' refers to the last name of the inventor, Edwin Southern. The word 'Blot' refers to the process of transferring the sample onto a membrane to be probed for the desired sequence. In IPA phonetic transcription, 'Southern' is /ˈsʌðərn/ and 'Blot' is /blɑt/. Together, the spelling is 'Sʌðərn blɑt'. The method has been widely used since its invention in 1975 and remains a powerful tool in genetics research.

SOUTHERN BLOT Meaning and Definition

1. Southern blot is a molecular biology technique used to detect specific DNA sequences in a sample. It was named after its inventor, Dr. Edwin Southern, and is predominantly employed to study gene presence, size, and abundance.

   The procedure involves several key steps. Initially, the DNA sample is extracted and purified. It is then digested with specific restriction enzymes, which cleave the DNA into smaller fragments. These fragments are then separated by size using gel electrophoresis, where an electric current is passed through a gel matrix, causing the DNA fragments to migrate based on their size.

   After electrophoresis, the DNA fragments are transferred or “blotted” onto a solid support, typically a membrane. This step involves capillary action or vacuum transfer techniques. The DNA fragments now immobilized on the membrane are then probed with a labeled DNA or RNA probe that is complementary to the target sequence of interest. The probe hybridizes specifically to the target DNA sequence and any excess probe is washed away.

   Finally, the immobilized DNA fragments are visualized using autoradiography or other detection methods, depending on the label used. This allows for the identification and analysis of the target DNA sequence in the original sample.

   Southern blotting is commonly employed for DNA fingerprinting, genetic testing, and the analysis of gene expression patterns. It has greatly contributed to our understanding of genetics and the identification of disease-causing mutations.

Common Misspellings for SOUTHERN BLOT

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Etymology of SOUTHERN BLOT

The term "Southern blot" is named after its inventor, Edwin M. Southern, who developed the technique in 1975. Southern blotting is a molecular biology technique used for detecting specific sequences of DNA in a DNA sample. The name was coined as a play on words, combining Southern's last name with the word "blotting", which refers to the transfer of DNA from a gel onto a solid support, typically a nitrocellulose membrane.

Similar spelling word for SOUTHERN BLOT

• southern blight.