Oligodeoxyribonucleotide Array Sequence Analysis is a mouthful of a term that refers to a technique used in genetics research. The IPA transcription of this word is /ɑːlɪgoʊˌdiːɒksirʌɪboʊnjuːkleɪətaɪd ˈærə/ which breaks down the pronunciation for each syllable. The term is spelled the way it is due to the scientific jargon that accompanies the field of genetics. It involves taking DNA molecules, breaking them apart, and reading their sequences to make new discoveries about the biological makeup of different organisms.
Oligodeoxyribonucleotide Array Sequence Analysis is a method used in molecular biology and genetics to analyze the presence and sequence of nucleic acids. It is commonly abbreviated as Oligoarray or OASIS. This technique involves the use of an array, which is a solid support material (such as a glass slide or a silicon chip) that is spotted with thousands of unique short DNA sequences known as oligonucleotides.
The purpose of Oligodeoxyribonucleotide Array Sequence Analysis is to determine the presence or expression levels of specific genes or sequences of interest within a sample. The array contains probes that are complementary to specific target sequences, allowing the identification and quantification of those sequences in the sample.
To perform Oligoarray, the sample DNA or RNA is isolated and labeled with a fluorescent tag. The labeled sample is then hybridized to the array, allowing the target sequences to bind to their specific complementary probes. The array is then scanned to detect the fluorescent signal emitted by the bound target sequences.
This technique has several applications, including gene expression profiling, single nucleotide polymorphism (SNP) analysis, and detection of genetic variations. It is widely used in research, diagnostics, and drug discovery. Oligodeoxyribonucleotide Array Sequence Analysis provides a high-throughput and cost-effective method for characterizing the DNA and RNA content of biological samples.