KOHLER ILLUMINATION Meaning and
Definition
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Kohler illumination is a technique used in microscopy to achieve even and optimum illumination of the specimen being observed. It is named after August Köhler, the inventor of this method. Kohler illumination is particularly important in high-performance light microscopy, as it ensures the highest image quality and contrast.
In Kohler illumination, light from an intense light source, such as a halogen lamp, is focused onto a condenser lens system. The light is then collimated into parallel rays and passes through an aperture diaphragm. The diaphragm can be adjusted to control the size and shape of the light beam. The light then continues to travel towards the specimen.
Next, the objective lens collects the light that has passed through the specimen. In Kohler illumination, the objective lens is positioned to form an image of the condenser aperture diaphragm on the specimen plane. This alignment ensures that the field of view is evenly illuminated and that the entire image is properly illuminated, regardless of the numerical aperture of the objective lens.
Kohler illumination also involves the use of field and aperture diaphragms in the microscope to further optimize the illumination. These diaphragms control the amount of light entering the objective lens and improve the contrast and resolution of the image.
Overall, Kohler illumination is a critical technique in modern microscopy, allowing for uniform and optimal illumination of specimens to achieve high-quality images.
Common Misspellings for KOHLER ILLUMINATION
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Etymology of KOHLER ILLUMINATION
The term "Kohler illumination" is named after its developer, August Köhler, a German physicist and microscopist. Köhler worked at the Carl Zeiss optics company in Germany in the late 1800s, where he developed a method to improve the illumination of microscopes. This method became known as "Kohler illumination" in honor of its creator. The term has since been widely used in microscopy to refer to the technique of achieving optimal illumination conditions for high-quality imaging.
