HAPII ENDONUCLEASE Meaning and
Definition
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HapII endonuclease, also known as HpaII endonuclease, is a type II restriction enzyme that recognizes specific DNA sequences and catalyzes the cleavage of the DNA at these recognition sites. It belongs to the family of restriction enzymes called methyltransferases, which are responsible for protecting bacterial DNA from the invasion of foreign DNA by cleaving it at specific locations.
The recognition sequence for HapII endonuclease is 5'-CCGG-3', meaning that it identifies a four-base pair sequence with cytosine at the first and second positions and guanine at the third and fourth positions. Once HapII endonuclease locates this specific recognition sequence, it breaks the phosphodiester bond between the second and third nucleotides, producing two DNA fragments with blunt ends.
HapII endonuclease exhibits strict specificity for its recognition sequence and is highly sensitive to DNA methylation at the cytosine residues within the recognition sequence. If any of the cytosines within the 5'-CCGG-3' sequence are methylated, HapII endonuclease is unable to cleave the DNA.
This restriction enzyme is widely used in molecular biology and genetic engineering techniques due to its ability to generate defined DNA fragments with blunt ends. It plays a crucial role in cloning experiments, gene mapping, and DNA sequencing, where DNA fragments are often manipulated and analyzed. Its specificity and versatility make HapII endonuclease a valuable tool in molecular biology research.
Common Misspellings for HAPII ENDONUCLEASE
- gapii endonuclease
- bapii endonuclease
- napii endonuclease
- japii endonuclease
- uapii endonuclease
- yapii endonuclease
- hzpii endonuclease
- hspii endonuclease
- hwpii endonuclease
- hqpii endonuclease
- haoii endonuclease
- halii endonuclease
- ha-ii endonuclease
- ha0ii endonuclease
- hapui endonuclease
- hapji endonuclease
- hapki endonuclease
- hapoi endonuclease
- hap9i endonuclease
- hap8i endonuclease
Etymology of HAPII ENDONUCLEASE
The word "HapII Endonuclease" is derived from the scientific nomenclature used for enzymes.
"HapII" refers to the bacterial strain from which the enzyme was initially isolated. In this context, the "Hap" element may signify the initials of the scientist(s) who discovered the enzyme or the name of the bacterial species it was isolated from. The Roman numeral "II" could denote a specific variant or subtype of the enzyme.
"Endonuclease" is a general term used for enzymes that are capable of breaking the phosphodiester bonds within the DNA molecule, specifically at certain points within the DNA chain. They are responsible for cleaving DNA at specific sequences, contributing to various biological processes such as DNA repair, recombination, and restriction digestion.
