EXON TRAPPING Meaning and
Definition
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Exon trapping refers to a molecular biology technique used to identify and isolate specific mRNA sequences encoding protein-coding exons within a genome. It relies on the ability to capture or "trap" the RNA transcripts produced from a gene and subsequently analyze them.
In this method, a genetic construct called an exon trap vector is constructed. The exon trap vector contains various regulatory elements necessary for transcription and translation. It also contains specific sequences or tags that enable the isolation and identification of the trapped mRNA. These sequences often include a reporter gene whose expression can be readily detected.
The exon trap vector is introduced into cells or organisms, where it integrates into the genome. If the cell or organism possesses a gene that is actively transcribed and produces mRNA, the exon trap vector can capture and retain the transcribed mRNA molecules through specific interactions.
After capturing the mRNA, the exon trap vector is extracted and analyzed. This analysis allows researchers to identify and characterize the trapped exons or coding regions. Exon trapping can reveal important information about the structure, splicing patterns, and function of genes. It can also aid in the discovery of novel genes, unraveling genetic mutations, and understanding gene regulation.
Overall, exon trapping is a valuable molecular biology technique that facilitates the elucidation of mRNA sequences encoding protein-coding exons, providing insights into gene structure and function.
Common Misspellings for EXON TRAPPING
- wxon trapping
- sxon trapping
- dxon trapping
- rxon trapping
- 4xon trapping
- 3xon trapping
- ezon trapping
- econ trapping
- edon trapping
- eson trapping
- exin trapping
- exkn trapping
- exln trapping
- expn trapping
- ex0n trapping
- ex9n trapping
- exob trapping
- exom trapping
- exoj trapping
- exoh trapping
Etymology of EXON TRAPPING
The term "exon trapping" is a compound word, derived from the words "exon" and "trapping". Here is the breakdown of their respective origins:
1. Exon: The word "exon" originated from the field of molecular biology and genetics. It was coined in the early 1980s as a short form of "expressed region". Exons are the coding regions of a gene that contain the necessary information for producing proteins. They are transcribed into messenger RNA (mRNA), which is then translated into a protein.
2. Trapping: The word "trapping" has its roots in Old English and Middle English. It originally referred to the act of capturing or ensnaring something, particularly animals, by means of a trap. Over time, the meaning of "trapping" expanded to include the sense of catching or capturing something, figuratively or literally.
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