ENDONUCLEASE AACI Meaning and
Definition
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Endonuclease AacI is a type II restriction enzyme that recognizes a specific DNA sequence and cleaves it at specific points. It is derived from the bacterium Aquifex aeolicus.
This enzyme belongs to the endonuclease subfamily that uses a magnesium-dependent mechanism to cut DNA molecules. Its recognition site consists of a palindromic DNA sequence, meaning it reads the same on both strands when the middle is inverted. Specifically, AacI recognizes and cleaves the sequence 5'-5'-GTGCAC-3'-3'. The cleavage occurs symmetrically within this sequence, resulting in two DNA fragments with blunt ends.
Endonuclease AacI plays a critical role in molecular biology research, especially in genetic engineering and recombinant DNA technology. It is frequently used in laboratory protocols for DNA fragment analysis, cloning, and DNA manipulation. By targeting and cleaving specific DNA sequences, AacI enables the precise manipulation of genetic material, allowing researchers to insert, delete, or modify DNA fragments as needed for various applications.
Due to its strict recognition sequence, AacI is highly specific in its DNA cleavage capabilities. This specificity makes it a valuable tool for molecular biologists in applications such as gene mapping, DNA sequencing, and genotyping. Additionally, its relatively small size and robust enzymatic activity make it practical and efficient for use in laboratory settings.
Common Misspellings for ENDONUCLEASE AACI
- wndonuclease aaci
- sndonuclease aaci
- dndonuclease aaci
- rndonuclease aaci
- 4ndonuclease aaci
- 3ndonuclease aaci
- ebdonuclease aaci
- emdonuclease aaci
- ejdonuclease aaci
- ehdonuclease aaci
- ensonuclease aaci
- enxonuclease aaci
- enconuclease aaci
- enfonuclease aaci
- enronuclease aaci
- eneonuclease aaci
- endinuclease aaci
- endknuclease aaci
- endlnuclease aaci
- endpnuclease aaci
Etymology of ENDONUCLEASE AACI
The word "endonuclease AacI" is a compound term composed of "endonuclease" and "AacI".
1. Endonuclease: The term "endonuclease" is derived from the combination of two Greek words, "endo" meaning "within" or "inside", and "nuclease" referring to an enzyme that cleaves nucleic acids. Therefore, "endonuclease" literally means an enzyme that cleaves nucleic acids internally or within the molecule.
2. AacI: "AacI" is a specific name assigned to a type of endonuclease based on the protocol used for classifying endonucleases. The naming scheme typically includes a three-letter abbreviation for the organism of origin followed by a Roman numeral indicating the order of discovery.
