ELECTROIMMUNOBLOTTINGS Meaning and
Definition
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Electroimmunoblottings, also known as immunoblotting or Western blotting, is a laboratory technique used to detect and analyze specific proteins within a complex protein mixture. This technique combines principles of electrophoresis and immunodetection.
The process of electroimmunoblottings involves several steps. Firstly, proteins from a biological sample are separated by size using gel electrophoresis. This separation occurs as a result of an electric current applied to the gel matrix. Subsequently, the separated proteins are transferred from the gel onto a solid membrane, usually composed of nitrocellulose or PVDF (polyvinylidene difluoride).
Following the transfer, the membrane is blocked to prevent non-specific binding of antibodies. Then, specific primary antibodies that recognize the target protein are applied, allowing them to bind to the protein of interest. After washing away unbound antibodies, a secondary antibody conjugated to an enzyme or fluorescent dye is introduced. The secondary antibody interacts with the primary antibody, producing a visual signal such as a colored band (enzyme-linked) or a fluorescent tag (fluorescent dye).
The resulting protein bands on the membrane can be detected and quantified using various methods, including enzymatic reactions or fluorescence detection systems. By comparing the protein band patterns obtained from different samples, scientists can determine the presence or absence of specific proteins and measure their relative abundance.
Overall, electroimmunoblottings provide researchers with a powerful tool to study protein expression and identify protein targets within a complex mixture, thus contributing to the understanding of disease mechanisms, protein interactions, and diagnostic applications in biomedical research.
Common Misspellings for ELECTROIMMUNOBLOTTINGS
- wlectroimmunoblottings
- slectroimmunoblottings
- dlectroimmunoblottings
- rlectroimmunoblottings
- 4lectroimmunoblottings
- 3lectroimmunoblottings
- ekectroimmunoblottings
- epectroimmunoblottings
- eoectroimmunoblottings
- elwctroimmunoblottings
- elsctroimmunoblottings
- eldctroimmunoblottings
- elrctroimmunoblottings
- el4ctroimmunoblottings
- el3ctroimmunoblottings
- elextroimmunoblottings
- elevtroimmunoblottings
- eleftroimmunoblottings
- eledtroimmunoblottings
- elecrroimmunoblottings
Etymology of ELECTROIMMUNOBLOTTINGS
The word "Electroimmunoblottings" is a compound word comprised of three main components:
1. Electro-: This prefix is derived from the Greek word "electron", which means amber. It is commonly used to refer to electricity or electric phenomena.
2. Immuno-: This prefix is derived from the Latin word "immunis", which means exempt. It is often related to immunity or immune responses.
3. Blotting: This word is derived from the verb "to blot", which refers to the act of absorbing or removing a substance. In the context of science, it specifically refers to a technique used to separate and identify proteins or nucleic acids.
Therefore, the term "Electroimmunoblottings" essentially describes a scientific technique that combines elements of electricity/electric phenomena, immune responses, and the process of separating and identifying proteins or nucleic acids.
