How Do You Spell DEOXYRIBONUCLEASE BSTI?

Pronunciation: [diːˈɒksɪɹˌɪbənjˌuːklɪˌe͡ɪs bˌiːˌɛstˈiː ˈa͡ɪ] (IPA)

The word "Deoxyribonuclease BstI" is spelled in a manner consistent with the International Phonetic Alphabet (IPA). The IPA is a standardized system of phonetic notation that represents the sounds of spoken language. In this case, the word starts with the "d" sound, followed by the long "ee" sound as in "see" and then "ox" as "ahx" followed by "ee" again. The "ro" sound is represented by "oh" followed by the "eye" sound as in "my". The rest of the word follows a similar pattern with "oo" as "ew" and "ah" as "a".

DEOXYRIBONUCLEASE BSTI Meaning and Definition

1. Deoxyribonuclease BstI is a type II restriction enzyme that specifically recognizes and cleaves DNA at specific nucleotide sequences. It is derived from Bacillus stearothermophilus and belongs to the family of endonucleases. BstI recognizes a palindromic sequence that consists of six nucleotides, 5ʹ-GGTAC↓C-3ʹ, where the arrow indicates the cleavage site. The enzyme cleaves the DNA strand at a specific position within this sequence, resulting in the generation of two DNA fragments with cohesive ends.

   Deoxyribonucleases, such as BstI, play a crucial role in molecular biology and genetic engineering techniques. They are widely used in recombinant DNA technology to manipulate DNA molecules. By specifically cleaving DNA at precise sites, restriction enzymes like BstI facilitate the construction of DNA fragments with desired sequences for various applications, including cloning, sequencing, and gene expression studies.

   Deoxyribonuclease BstI exhibits optimal activity at temperatures around 60-65 degrees Celsius, making it a thermostable enzyme that is resistant to heat denaturation. This property is advantageous for applications requiring elevated temperatures, such as PCR amplification of DNA fragments.

   In summary, Deoxyribonuclease BstI is an endonuclease derived from Bacillus stearothermophilus that recognizes a specific DNA sequence and cleaves it at a precise site. It is commonly used in molecular biology research and genetic engineering techniques for DNA manipulation and analysis.

Common Misspellings for DEOXYRIBONUCLEASE BSTI

• seoxyribonuclease bsti
• xeoxyribonuclease bsti
• ceoxyribonuclease bsti
• feoxyribonuclease bsti
• reoxyribonuclease bsti
• eeoxyribonuclease bsti
• dwoxyribonuclease bsti
• dsoxyribonuclease bsti
• ddoxyribonuclease bsti
• droxyribonuclease bsti
• d4oxyribonuclease bsti
• d3oxyribonuclease bsti
• deixyribonuclease bsti
• dekxyribonuclease bsti
• delxyribonuclease bsti
• depxyribonuclease bsti
• de0xyribonuclease bsti
• de9xyribonuclease bsti
• deozyribonuclease bsti
• deocyribonuclease bsti

Etymology of DEOXYRIBONUCLEASE BSTI

The word "Deoxyribonuclease BstI" is made up of several components:

1. Deoxyribonuclease: The term "deoxyribonuclease" is derived from several parts. "Deoxyribo-" refers to deoxyribose, a sugar molecule found in DNA. "Nuclease" comes from the Latin word "nuclease" which means an enzyme that breaks down nucleic acids.

2. BstI: "BstI" is an abbreviation for the restriction enzyme derived from Bacillus stearothermophilus, which was discovered in the laboratory of Werner Arber. It is named after the initials of the organism from which it was isolated.

Similar spelling word for DEOXYRIBONUCLEASE BSTI

• BstI Deoxyribonuclease.