COLONY HYBRIDISATION Meaning and
Definition
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Colony hybridization is a molecular biology technique used to detect specific DNA or RNA sequences within a large population of microorganisms or cells. It involves the transfer of DNA or RNA from a colony of microorganisms onto a solid support, followed by hybridization with a complementary labeled probe.
In this technique, a population of microorganisms is grown on an agar plate until individual colonies are formed, each derived from a single cell. These colonies can potentially contain cells with specific DNA or RNA sequences of interest. The colonies are then transferred onto a solid support, such as a nitrocellulose membrane or nylon filter, using a process called replica plating.
Once the colonies are immobilized on the membrane, it is subjected to hybridization. A specific labeled probe, either DNA or RNA, that is complementary to the target sequence of interest is applied to the membrane. The probe binds specifically to its complementary sequence within the colonies, forming a stable duplex.
After hybridization, any unbound probe is washed away, and the membrane is exposed to a detection method, such as autoradiography or chemiluminescence, depending on the label used. This allows the detection and visualization of colonies that contain the target sequence.
Colony hybridization is widely used in the fields of microbiology and genetics for screening and identifying DNA or RNA sequences of interest, such as genes or specific microorganisms within a population. It provides a powerful tool for studying the presence or absence of specific genetic material in a large number of samples simultaneously.
Common Misspellings for COLONY HYBRIDISATION
- xolony hybridisation
- volony hybridisation
- folony hybridisation
- dolony hybridisation
- cilony hybridisation
- cklony hybridisation
- cllony hybridisation
- cplony hybridisation
- c0lony hybridisation
- c9lony hybridisation
- cokony hybridisation
- copony hybridisation
- cooony hybridisation
- coliny hybridisation
- colkny hybridisation
- collny hybridisation
- colpny hybridisation
- col0ny hybridisation
- col9ny hybridisation
- coloby hybridisation
Etymology of COLONY HYBRIDISATION
The word "colony hybridisation" consists of two main parts: "colony" and "hybridisation".
1. Colony: The word "colony" comes from the Latin word "colonia", which originally referred to a settlement or territory established by ancient Romans outside of Italy. Over time, the term extended its meaning to describe a group of people or animals living together in a specific area. In the context of microbiology, a colony describes a visible cluster of microbial cells that have grown and multiplied on a solid culture medium.
2. Hybridisation: "Hybridisation" derives from the verb "hybridize", which emerged in the early 19th century. The term "hybrid" originated from the Latin word "hybridus", meaning "mongrel" or "offspring of mixed parentage".
