CLELANDS REAGENT Meaning and
Definition
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Cleland's reagent refers to a specific chemical compound that is commonly used in biochemistry and molecular biology research. It is named after its creator, William D. Cleland, who developed this reagent in the 1960s. Cleland's reagent is primarily utilized for the quantification and study of enzymatic activities, particularly the measurement of enzyme kinetics.
The chemical composition of Cleland's reagent consists of a pyridinium ring, a thiol group, and a short aliphatic chain. It is typically synthesized by reacting iodoacetate with pyridine, resulting in the formation of the active pyridinium derivative. The thiol group in Cleland's reagent is responsible for covalently modifying the active site of enzymes by reacting with the active cysteine residues of the enzyme, thus irreversibly inactivating it.
This reagent is commonly employed in Michaelis-Menten enzyme kinetic studies to determine parameters such as the maximal velocity (Vmax) and the Michaelis constant (Km) of enzymatic reactions. The irreversible nature of Cleland's reagent ensures that once the enzyme is modified, it remains inactive throughout the duration of the experiment.
Overall, Cleland's reagent is an invaluable tool in enzymology research as it provides a means of characterizing and quantifying enzyme activities. Its ability to irreversibly modify the active site of enzymes allows for accurate and reproducible measurements of enzymatic parameters, contributing to a better understanding of biological processes and facilitating the development of new therapeutic strategies.
Common Misspellings for CLELANDS REAGENT
- xlelands reagent
- vlelands reagent
- flelands reagent
- dlelands reagent
- ckelands reagent
- cpelands reagent
- coelands reagent
- clwlands reagent
- clslands reagent
- cldlands reagent
- clrlands reagent
- cl4lands reagent
- cl3lands reagent
- clekands reagent
- clepands reagent
- cleoands reagent
- clelznds reagent
- clelsnds reagent
- clelwnds reagent
- clelqnds reagent
Etymology of CLELANDS REAGENT
The word "Clelands Reagent" is named after George Cleland, an Australian chemist who developed the reagent. The term "reagent" generally refers to a substance or compound used in chemical reactions to detect, measure, or produce other substances. In this case, Clelands Reagent is specifically used as a tool to investigate protein structure and function through protein modification.
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