CLELAND REAGENT Meaning and
Definition
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Cleland Reagent is a specific chemical compound used in biochemistry and molecular biology for the study of peptide and protein structures. It is a synthetic reagent that is often employed to reduce or break peptide and disulfide bonds in proteins, aiding in the analysis and characterization of these molecules.
The Cleland Reagent, named after the American biochemist Robert Cleland, typically consists of a reducing agent, commonly dithiothreitol (DTT) or beta-mercaptoethanol, dissolved in a suitable buffer solution. These reagents are known for their ability to break disulfide bonds, which are sulfur-sulfur bonds found in proteins that contribute to their three-dimensional structure and stability.
The addition of Cleland Reagent to a protein sample can result in the reduction of disulfide bonds, leading to the formation of sulfhydryl groups (-SH). This modification allows for further analysis, such as protein fragmentation or the determination of the number and location of disulfide bonds in the protein structure.
Cleland Reagent is widely used in various experimental techniques, including protein sequencing, protein folding studies, and protein structure determination by X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy. Furthermore, it can be employed to monitor the effects of oxidative stress on proteins and investigate protein-protein interactions by selectively modifying specific amino acids.
In summary, Cleland Reagent is a specialized chemical compound used to reduce or break disulfide bonds in proteins, aiding in the analysis and characterization of their structure and function in the field of biochemistry and molecular biology.
Common Misspellings for CLELAND REAGENT
- xleland reagent
- vleland reagent
- fleland reagent
- dleland reagent
- ckeland reagent
- cpeland reagent
- coeland reagent
- clwland reagent
- clsland reagent
- cldland reagent
- clrland reagent
- cl4land reagent
- cl3land reagent
- clekand reagent
- clepand reagent
- cleoand reagent
- clelznd reagent
- clelsnd reagent
- clelwnd reagent
- clelqnd reagent
Etymology of CLELAND REAGENT
The word "Cleland Reagent" is derived from the name of the American biochemist, James Ralph Cleland, who developed this specific reagent. James Cleland is known for his contributions to the field of protein chemistry and enzymology. The reagent he developed is used for studying and modifying proteins and peptides by selectively cleaving disulfide bonds, which are important for protein structure and stability. The term "Reagent" refers to a substance or compound used in a chemical reaction to detect, measure, analyze, or modify other substances.
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