bDNA Signal Amplification Assay, also known as Branched DNA Signal Amplification Assay, is a laboratory technique used to accurately quantify specific nucleic acid targets, such as DNA or RNA, in a biological sample.
The bDNA Signal Amplification Assay is based on the unique branched DNA molecule, which consists of multiple target-specific probes branching from a common backbone. The assay involves several steps to amplify the signal, providing a highly sensitive and specific detection method.
In this assay, the target nucleic acid in the sample is first captured by specific capture probes immobilized on a solid support. Next, signal amplification is achieved by hybridizing several target-specific bDNA probes to the captured target molecules. Each of these bDNA probes contains multiple branches that can be bound by pre-amplifier molecules, which in turn bind to amplifier molecules. The amplifier molecules are attached to chemiluminescent labels that generate detectable signals.
The bDNA Signal Amplification Assay allows for quantification of the target nucleic acids without the need for polymerase chain reaction (PCR), enhancing its specificity and eliminating the risk of amplification bias. The amplified signal is directly proportional to the amount of target nucleic acid present in the sample, enabling accurate quantification.
This assay has proven valuable in various fields, including clinical diagnostics, infectious disease monitoring, genetic research, and drug discovery. Its high sensitivity, specificity, and ease of use make it a preferred choice for quantitative nucleic acid analysis.
