BamKI Endonuclease is a bioengineering term that refers to a type of enzyme used in molecular biology. The word "BamKI" is derived from the two bacteria from which the enzyme was isolated, Bacillus amyloliquefaciens and Klebsiella pneumoniae. The IPA phonetic transcription of the word would be [bæmkæɪ ɛndounjuːkleɪs]. The "Bam" sound is pronounced like "bam" in "bamboo," and the "KI" sound is pronounced like "key." The stress is placed on the "KI" syllable, indicating its importance.
BamKI Endonuclease refers to a type II restriction endonuclease enzyme isolated from the Bacillus amyloliquefaciens strain H. It is known for its ability to cleave DNA at specific sites with high precision and efficiency.
This endonuclease recognizes a particular DNA sequence known as a recognition site, which consists of a palindromic sequence. In the case of BamKI, the recognition site is 5'-GGATCC-3'. The recognition sequence is present on both strands of DNA and is symmetrical, meaning it reads the same from both directions.
Once the BamKI enzyme identifies its recognition site, it cleaves the DNA double helix in a precise manner, resulting in the formation of two DNA fragments with sticky ends. Sticky ends refer to DNA fragments that have single-stranded overhangs known as cohesive ends. In the case of BamKI, the cohesive ends are complementary to each other, as they are derived from the palindromic sequence of the recognition site.
The cleavage activity of BamKI makes it a valuable tool for molecular biology research, especially in the field of genetic engineering. Researchers utilize this enzyme to manipulate DNA molecules, such as cutting and pasting specific DNA fragments into other sequences, creating recombinant DNA molecules. Furthermore, it aids in the study of gene expression, protein production, and the investigation of specific genetic sequences in various organisms.
The word "BamKI" is derived from the name of the bacterium from which it was first discovered and isolated - Bacillus amyloliquefaciens strain H. The letter "K" represents the strain designated as B. amyloliquefaciens H K. "BamKI" is a combination of the first three letters of the genus name (Bam) and the first letter of the species name (K) of the bacterium.
The term "endonuclease" refers to the function of the protein as an enzyme that cleaves the DNA chain at specific nucleotide sequences. In this case, BamKI is an endonuclease that recognizes a particular DNA sequence and cuts the chain at that specific site.