How Do You Spell BAMHI ENDONUCLEASE?

Pronunciation: [bˈam ˌe͡ɪt͡ʃˈa͡ɪ ˈɛndə͡ʊnjˌuːklɪˌe͡ɪs] (IPA)

The correct spelling of the word "BamHI Endonuclease" is pronounced as [bæm.eɪtʃ.aɪ ɛndoʊ.nju.kli:iz]. The "Bam" in BamHI is pronounced with a short "a" sound followed by an "m" sound. The "H" is pronounced as an "h" sound while the "I" is pronounced as "ai". Endonuclease is pronounced as [ɛndoʊ.nju.kli:iz]. The "e" is pronounced with a short "e" sound and the "o" is pronounced as "oh". The "u" is pronounced as "yu" and the "c" is pronounced as an "s" sound.

BAMHI ENDONUCLEASE Meaning and Definition

1. BamHI endonuclease is a commonly used type II restriction enzyme that is obtained from the bacterium Bacillus amyloliquefaciens H. It belongs to the restriction-modification system, where it acts as an endonuclease by recognizing specific DNA sequences and cleaving them at specific points.

   BamHI recognizes the DNA sequence 5'-GGATCC-3' and cleaves it between the two G nucleotides, generating cohesive or sticky ends. The cohesive ends are complementary to each other, allowing for the specific joining or ligation with other DNA fragments that have been digested with BamHI or a compatible restriction enzyme.

   This endonuclease is widely used in molecular biology applications, particularly in genetic engineering and cloning techniques. Its ability to precisely cleave DNA at specific sites allows researchers to manipulate and rearrange DNA sequences, aiding in the insertion or removal of specific genes or segments of DNA.

   The cleavage activity of BamHI endonuclease is dependent on various factors such as divalent cations, pH, and temperature. It functions optimally in reaction buffer composed of magnesium ions at a pH of around 7-8.

   BamHI and other restriction enzymes are essential tools in DNA manipulation techniques, enabling the generation of recombinant DNA molecules and facilitating the study of gene function, protein expression, and genetic engineering.

Common Misspellings for BAMHI ENDONUCLEASE

• vamhi endonuclease
• namhi endonuclease
• hamhi endonuclease
• gamhi endonuclease
• bzmhi endonuclease
• bsmhi endonuclease
• bwmhi endonuclease
• bqmhi endonuclease
• banhi endonuclease
• bakhi endonuclease
• bajhi endonuclease
• bamji endonuclease
• bamui endonuclease
• bamyi endonuclease
• bamhu endonuclease
• bamhj endonuclease
• bamhk endonuclease
• bamho endonuclease
• bamh9 endonuclease
• bamh8 endonuclease

Etymology of BAMHI ENDONUCLEASE

The word BamHI derives its etymology from the name of the bacterium from which it was originally derived. It is named after the bacterium Bacillus amyloliquefaciens strain H, from which the enzyme was first isolated and characterized. The name BamHI combines the first three letters of the genus name Bacillus with the first two letters of the species name amyloliquefaciens and the first letter of the strain name H. The term endonuclease refers to the function of the enzyme as an enzyme that cleaves DNA at specific recognition sites internally (within the DNA sequence), hence the term endonuclease.