ACDPCR stands for Absolute Copy Depth–Corrected Polymerase Chain Reaction. It is a molecular biology technique that involves quantifying the absolute copy number of a specific DNA sequence or gene of interest. The method combines the principles of polymerase chain reaction (PCR) amplification and copy number variation (CNV) analysis.
In ACDPCR, genomic DNA or cDNA is first extracted from cells or tissues. Then, a specific region of interest is amplified using PCR with target-specific primers. This amplification step exponentially increases the DNA molecules corresponding to the gene of interest.
After the PCR amplification, the resulting DNA products are quantified using techniques such as qPCR (quantitative polymerase chain reaction) or digital PCR. These quantification methods provide accurate information about the absolute copy number of the targeted DNA sequence.
To correct for potential variations in DNA concentration and amplification efficiency between samples, a reference gene or an internal control with a known copy number is used. By normalizing the copy number of the gene of interest to the internal control, the ACDPCR technique allows researchers to accurately determine the absolute copy number of the target gene in a sample.
ACDPCR finds applications in various fields, including research on gene expression, genetic disorders, DNA diagnostics, and oncology. The accurate quantification of DNA copy numbers aids in understanding disease mechanisms, identifying prognostic markers, and evaluating potential therapeutic targets.
