TWO DIMENSIONAL GEL ELECTROPHORESIS Meaning and
Definition
-
Two-dimensional gel electrophoresis (2D gel electrophoresis) is a powerful technique in the field of proteomics, used to separate and analyze complex mixtures of proteins. It allows the separation of proteins based on their isoelectric point (pI) and molecular weight, providing a highly detailed analysis of the proteome.
In two-dimensional gel electrophoresis, the first dimension involves the separation of proteins based on their isoelectric points. A protein sample is loaded onto a gel strip with a pH gradient, and an electric field is applied. As the proteins migrate through the strip, they focus at their respective pI points.
The second dimension utilizes SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) to separate the proteins based on their molecular weight. The focused proteins from the first dimension are then transferred onto an SDS-PAGE gel and an electric field is applied again. This separation allows a visual representation of the protein components in a sample, forming a two-dimensional gel.
Following electrophoresis, the two-dimensional gel can be stained to visualize the separated proteins. Different protein spots on the gel represent distinct protein species. These protein spots can then be excised from the gel for further analysis like protein identification using mass spectrometry.
Two-dimensional gel electrophoresis is widely used in proteomic studies to compare protein expression patterns between different samples, such as healthy and diseased tissues or cells exposed to different treatments. It provides a comprehensive and high-resolution analysis of protein mixtures, contributing to the understanding of complex biological systems.
Common Misspellings for TWO DIMENSIONAL GEL ELECTROPHORESIS
- rwo dimensional gel electrophoresis
- fwo dimensional gel electrophoresis
- gwo dimensional gel electrophoresis
- ywo dimensional gel electrophoresis
- 6wo dimensional gel electrophoresis
- 5wo dimensional gel electrophoresis
- tqo dimensional gel electrophoresis
- tao dimensional gel electrophoresis
- tso dimensional gel electrophoresis
- teo dimensional gel electrophoresis
- t3o dimensional gel electrophoresis
- t2o dimensional gel electrophoresis
- twi dimensional gel electrophoresis
- twk dimensional gel electrophoresis
- twl dimensional gel electrophoresis
- twp dimensional gel electrophoresis
- tw0 dimensional gel electrophoresis
- tw9 dimensional gel electrophoresis
- two simensional gel electrophoresis
- two ximensional gel electrophoresis
