TAILPCR Meaning and
Definition
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TAIL-PCR refers to Template-Amplification of Integrated LDSs by PCR. This is a molecular biology technique used to specifically amplify DNA fragments that are adjacent to a known DNA sequence. TAIL-PCR is primarily employed to isolate the flanking regions of known transposon or retrovirus insertions in model organisms. It relies on the concept that transposon or retrovirus insertions generate repetitive sequences at both ends of the integrated DNA fragment. By exploiting these repetitive sequences as targets for PCR amplification, TAIL-PCR allows for the identification and cloning of the unknown genomic sequences adjacent to the insertion site.
The TAIL-PCR method involves a series of nested PCRs that use nested primers specific to the known DNA sequence and primer walking with arbitrary degenerate primers that anneal to the repetitive sequences generated by transposon or retrovirus insertions. The specificity and efficiency of TAIL-PCR amplification is determined by the strict design of the nested primers and the concentration of the degenerate primers.
TAIL-PCR has broad applications in genomic research, particularly in the identification and characterization of genes affected by transposon or retrovirus insertions. It allows researchers to investigate gene function and regulation by determining the genomic context of these insertions. The technique also enables the construction of genetic maps and the development of molecular markers for genome analysis.
Common Misspellings for TAILPCR
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