Subcellular fractions refer to the different parts of a cell that can be separated, for example by centrifugation. The word "subcellular" is pronounced /ˌsʌbseljʊlər/, with the emphasis on the second syllable. It is spelled "sub-" as in "submarine" and "cellular" as in "cell". "Fractions" is pronounced /ˈfrækʃənz/ and is spelled as it sounds, with the "-tion" suffix denoting a noun form. These fractions can provide essential information about the structure and function of a cell, and are widely used in research.
Subcellular fractions refer to the specific components or organelles that are obtained by separating different parts of a cell. This process involves the physical or biochemical isolation of different cellular elements, allowing for the study and analysis of their unique characteristics and functions. These fractions are primarily obtained through cell fractionation techniques, which employ methods such as ultracentrifugation, cell disruption, differential centrifugation, density gradient centrifugation, or immunoprecipitation.
Subcellular fractions are essential tools in various areas of cell biology and biochemistry. They enable researchers to separate and concentrate specific organelles or cell components, such as mitochondria, nuclei, ribosomes, lysosomes, peroxisomes, or microsomes, from complex mixtures.
The isolated subcellular fractions have distinctive biochemical compositions and functions. By analyzing these fractions, scientists can investigate the molecular interactions, metabolic pathways, and physiological role of the isolated organelles. This approach helps to gain insights into the mechanisms underlying cellular processes, including energy production, protein synthesis, secretion, degradation, or signal transduction.
Moreover, subcellular fractions facilitate the purification and separation of biomolecules, such as proteins or nucleic acids, present within the respective organelles. This allows for further detailed analysis and characterization of specific molecules. Subcellular fractionation techniques play a vital role in molecular biology research, allowing scientists to delve into the intricacies of cellular structure and function.
The word "subcellular" is derived from the combination of two roots - "sub" and "cellular". The prefix "sub" means "below" or "under", indicating that something is situated beneath or within another entity. "Cellular" refers to cells, the basic structural and functional units of living organisms.
The term "fractions" in this context refers to a portion or a part that has been separated or divided from a whole. Thus, "subcellular fractions" refers to the different parts or components obtained by breaking down or isolating specific regions or organelles within a cell.