Splicing is spelled with the letters s-p-l-i-c-i-n-g. In IPA phonetic transcription, splicing is pronounced as /ˈsplʌɪsɪŋ/. The word starts with the consonant blend /spl/, followed by the diphthong /ʌɪ/ and ends with the consonant sound /ŋ/. Splicing refers to joining two pieces of material, such as wires or film, by overlapping and binding them together. It is commonly used in a variety of industries, including engineering, construction, and film-making.
Splicing is a term commonly used in genetics and molecular biology to describe the process of joining together segments of DNA, RNA, or proteins. It involves the cutting and rejoining of these segments to form a complete and functional molecule.
In genetics, splicing typically refers to the removal of introns, which are noncoding regions within a gene, from the precursor messenger RNA (pre-mRNA). This process, known as RNA splicing, allows the production of a mature mRNA molecule that can be translated into a protein. The removal of introns and joining of exons (the coding regions) are achieved by a complex molecular machinery called the spliceosome.
Similarly, in molecular biology, splicing can also refer to the cutting and joining of DNA segments. This process is essential in recombinant DNA technology, where DNA segments from different sources are combined to create a modified DNA molecule with desired traits.
In the context of proteins, splicing can occur during the synthesis of a polypeptide chain. It involves the removal of specific amino acid sequences, called inteins, from within a protein, followed by joining the remaining sequences, known as exteins. Protein splicing is a rare phenomenon observed in certain proteins, and it can result in the production of multiple protein variants from a single gene.
Overall, splicing is a fundamental process that plays a critical role in the regulation of gene expression and the generation of diversity in genetic material.
The word "splicing" comes from the Middle English word "splissen" or "splisen", which can be traced back to the Old Norse word "splissa". This Old Norse word means "to split" or "to cleave", and it ultimately derives from the Proto-Germanic root "splītaną". The word "splicing" originally referred to the act of joining two pieces of rope or other flexible material by intertwining or interweaving their individual strands. Over time, the term has expanded in usage to include joining various other materials or components together in a similar manner.