Sequence Tagged Site (STS) is a specific type of marker used in molecular biology and genetics research to identify and map genes or specific regions of DNA sequences. It is a short, unique DNA fragment that is present only once in the genome and can be easily detected using polymerase chain reaction (PCR) techniques.
In a genomic sequence, an STS acts as a recognizable landmark, providing a known reference point for genetic mapping and sequencing purposes. It is typically characterized by a unique DNA sequence, which can be amplified and visualized using PCR and subsequent gel electrophoresis or other detection methods.
STS markers are employed in genetic linkage analysis studies to track the inheritance pattern of specific genes or genetic traits across generations. By locating and tracking the presence or absence of STS markers within a DNA sample or population, researchers can determine the genetic distance and relative location of genes or gene variants.
Moreover, STS markers play a significant role in physical mapping techniques, aiding in the identification and characterization of genetic landmarks on chromosomes. They facilitate the ordering, alignment, and orientation of overlapping clones in the construction of physical maps, enabling the assembly and annotation of entire genomes.
In summary, a Sequence Tagged Site (STS) is a distinctive DNA sequence used as a marker to locate and map genes or specific regions of interest within a genome, providing critical information for genetic linkage analysis and physical mapping.
