SEHPLC is an acronym that stands for Supercritical Fluid Extraction with High-Performance Liquid Chromatography. This term refers to a specialized scientific technique that combines two distinct processes – supercritical fluid extraction and high-performance liquid chromatography – for the purpose of extracting and analyzing compounds.
Supercritical fluid extraction (SFE) is a process that utilizes supercritical fluids as the solvent to extract target compounds from a sample. Supercritical fluids are substances that are maintained at temperatures and pressures above their critical points, where they possess unique properties that make them suitable solvents for extraction. By using a supercritical fluid, such as carbon dioxide, as the extracting solvent, the technique allows for the selective extraction of compounds in a sample.
High-performance liquid chromatography (HPLC) is a widely used analytical method that involves separating, identifying, and quantifying different components in a mixture. HPLC uses a stationary phase and a mobile phase, often a liquid solvent, to separate and carry the sample components through a column. Various detection techniques, such as UV or mass spectrometry, are employed to analyze the separated components.
SEHPLC combines the advantages of both supercritical fluid extraction and high-performance liquid chromatography. It utilizes supercritical fluids as the extracting solvent and subsequently separates and analyzes the extracted compounds using HPLC. This enables the extraction and analysis of a broad range of compounds, providing valuable information about the composition of a sample. SEHPLC is an important tool in various fields, including pharmaceutical, environmental, and food sciences, where the identification and quantification of compounds are crucial for research, quality control, and regulatory purposes.
