How Do You Spell SDS-PAGE?

Pronunciation: [ˌɛsdˌiːˈɛspˈe͡ɪd͡ʒ] (IPA)

The term "sds-page" refers to a technique of gel electrophoresis commonly used in biochemistry. The spelling of "sds-page" can be explained using IPA phonetic transcription as "ɛs.di.ɛs. peɪdʒ," where "ɛs" represents the sound of the letter "S," "di" represents the sound of the letter "D," and "peɪdʒ" represents the sound of the word "page." The use of IPA helps to provide a clear and standardized way of understanding the pronunciation and spelling of scientific terms.

SDS-PAGE Meaning and Definition

  1. SDS-PAGE, or sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is a widely used technique in biochemistry and molecular biology to separate and analyze proteins based on their molecular weight. It is primarily employed for denaturing and resolving proteins from a mixture, allowing for their identification and characterization.

    During SDS-PAGE, proteins are first denatured by adding an anionic detergent called sodium dodecyl sulfate (SDS) to unfolded proteins. SDS binds to proteins in a ratio of 1.4 grams of SDS per gram of protein, imparting a negative charge to each protein molecule in proportion to its length. This enables separation of proteins based on their molecular weight as they will migrate towards the positive anode in the electrophoresis gel at a rate determined by their charge-to-mass ratio.

    The polyacrylamide gel used in SDS-PAGE acts as a molecular sieve, with pores of different sizes that create a sieving effect. Smaller proteins move more rapidly through the gel matrix, while larger proteins move more slowly. Consequently, SDS-PAGE facilitates the separation of proteins into discrete bands that can be visualized following staining or transfer onto a membrane for immunoblotting.

    The separated proteins can be further analyzed through various techniques, such as western blotting or mass spectrometry, to identify and quantify specific proteins of interest. Additionally, the intensity of the bands on the gel can provide relative information about the abundance of different proteins within the original sample.

    Overall, SDS-PAGE is an essential tool in protein research and biotechnology, allowing for the separation, identification, and analysis of proteins in a mixture based on their molecular weight.

Common Misspellings for SDS-PAGE

  • sds pages
  • sd page
  • sds pge
  • sds pag
  • sds page
  • SDS PAGa
  • sdspages
  • sds pagae
  • ssdspage

Etymology of SDS-PAGE

The term "SDS-PAGE" is an abbreviation that stands for "sodium dodecyl sulfate-polyacrylamide gel electrophoresis".

The word "sodium dodecyl sulfate" refers to a detergent that plays a crucial role in this analytical technique. SDS is used to denature and solubilize proteins, giving them a negative charge and allowing them to separate based on their size during electrophoresis.

"Polyacrylamide gel electrophoresis" refers to the use of a gel made from polyacrylamide, a synthetic polymer, as the medium in which the protein separation takes place. Electrophoresis refers to the movement of charged particles in an electric field.

Therefore, the term "SDS-PAGE" is a combination of the abbreviations for the key components of the technique and the type of gel used.

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