RSIPCR Meaning and
Definition
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RSIPCR stands for Reverse Transcriptase-Strand Invasion Based Polymerase Chain Reaction. It is a molecular biology technique that combines elements of reverse transcription and the polymerase chain reaction (PCR) to amplify specific RNA sequences of interest.
Reverse transcription is the process of synthesizing complementary DNA (cDNA) from a template of RNA. During RSIPCR, this step is facilitated by the enzyme reverse transcriptase, which produces cDNA using a primer that binds to the target RNA sequence. The resulting cDNA can then be used as a template for PCR.
Strand invasion is an important characteristic of RSIPCR. It involves the binding of a single-stranded DNA oligonucleotide, called an invader, to its complementary sequence within the cDNA. This step leads to the formation of a stable DNA:DNA duplex. The invader is designed to contain a primer binding site for subsequent PCR amplification.
The polymerase chain reaction is used to selectively amplify the target cDNA sequence. An enzyme called DNA polymerase synthesizes new DNA strands that are complementary to the cDNA template. This process is repeated multiple times, resulting in the exponential amplification of the target sequence.
RSIPCR has potential applications in various areas of molecular biology, including gene expression analysis, detection of viral RNA, and identification of rare RNA molecules. Its unique combination of reverse transcription and strand invasion offers advantages such as increased sensitivity, specificity, and robustness compared to traditional PCR methods.
Common Misspellings for RSIPCR
- dsipcr
- tsipcr
- 5sipcr
- 4sipcr
- raipcr
- rzipcr
- rxipcr
- rdipcr
- rwipcr
- rsupcr
- rsjpcr
- rskpcr
- rsopcr
- rs9pcr
- rs8pcr
- rsiocr
- rsilcr
- rsi-cr
- rsi0cr
- rsipxr
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