PROTEINASE K Meaning and
Definition
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Proteinase K is a highly specific serine protease enzyme that belongs to the subfamily of subtilisin-like serine proteases. It is derived from the fungus Tritirachium album. Proteinase K is widely used in molecular biology and biochemistry research due to its remarkable ability to cleave peptide bonds at the carboxylic side of aromatic, hydrophobic, and neutral amino acids. This enzyme displays exceptional resistance to denaturing agents such as detergents, chaotropic agents, and even high temperatures, making it particularly valuable for protein digestion under harsh conditions.
Proteinase K has a broad substrate specificity, meaning it can cleave a wide range of proteins and peptides. Its preference for cleaving peptide bonds adjacent to hydrophobic amino acids makes it especially effective for removing unwanted proteins, nucleases, or other enzymes in various applications, such as DNA and RNA purification or the preparation of specific protein fragments for structural studies.
With its robust properties, Proteinase K has become an essential tool in molecular biology research, where it is used for DNA extraction, gene expression analysis, protein degradation studies, removal of contaminants, and in situ hybridization procedures. Its versatility and efficiency have made it a staple in laboratories worldwide, enabling scientists to accomplish a plethora of important experimental procedures across a wide array of scientific disciplines.
Common Misspellings for PROTEINASE K
- oroteinase k
- lroteinase k
- -roteinase k
- 0roteinase k
- peoteinase k
- pdoteinase k
- pfoteinase k
- ptoteinase k
- p5oteinase k
- p4oteinase k
- priteinase k
- prkteinase k
- prlteinase k
- prpteinase k
- pr0teinase k
- pr9teinase k
- proreinase k
- profeinase k
- progeinase k
- proyeinase k
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