POLYLIGATION Meaning and
Definition
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Polyligation is a term used in the field of genetic engineering and biotechnology, specifically in relation to the manipulation of DNA molecules and their fragments. It refers to the process of joining together multiple DNA fragments into a larger, more complex molecule. This technique is widely employed in molecular biology research, gene cloning, and recombinant DNA technology.
During the polyligation process, specific enzymes, such as DNA ligases, are used to catalyze the formation of covalent bonds between adjacent DNA fragments. By combining multiple DNA fragments, scientists can create novel genetic constructs, such as plasmids or artificial chromosomes, that can then be incorporated into host organisms for further study or application. Polyligation is a vital step in various molecular biology techniques, such as gene sequencing, gene expression analysis, and the creation of genetic libraries.
Polyligation is integral to the process of recombinant DNA technology, where the desired gene or DNA fragment is combined with a vector, often a plasmid or viral genome. This enables the replication and expression of the inserted DNA sequence in host organisms. By utilizing polyligation, researchers can construct complex and functional genetic entities, allowing for the manipulation and analysis of genes and their regulatory elements.
In summary, polyligation is the procedure of joining multiple DNA fragments together using enzymes, allowing scientists to create complex genetic constructs and enable further study or utilization of specific genes and DNA sequences.
Common Misspellings for POLYLIGATION
- oolyligation
- lolyligation
- -olyligation
- 0olyligation
- pilyligation
- pklyligation
- pllyligation
- pplyligation
- p0lyligation
- p9lyligation
- pokyligation
- popyligation
- pooyligation
- poltligation
- polgligation
- polhligation
- poluligation
- pol7ligation
- pol6ligation
- polykigation
Etymology of POLYLIGATION
The word "polyligation" is a combination of two components: "poly-" and "ligation".
1. The prefix "poly-" is derived from the Greek word "polus", meaning "many" or "much". When used as a prefix in English, "poly-" indicates something that involves or relates to many or multiple aspects.
2. The term "ligation" comes from the Latin word "ligare", which means "to bind" or "to tie". In English, "ligation" typically refers to the process of tying or binding something, such as a surgical procedure involving the closure or sealing of blood vessels or ducts using sutures, clips, or other mechanical means.
Therefore, the word "polyligation" suggests a procedure or process involving the binding or tying of multiple structures or aspects together.
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