PCRD Meaning and
Definition
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PCRD stands for Polymerase Chain Reaction with Reverse Transcriptase. It is a laboratory technique used in molecular biology, which combines two different processes: reverse transcription and polymerase chain reaction (PCR). PCRD is primarily employed in the study of gene expression and analysis of RNA molecules.
Reverse transcription is the process of converting RNA into complementary DNA (cDNA), using the enzyme reverse transcriptase. This enzyme synthesizes a single-stranded cDNA from an RNA template molecule. PCRD involves the reverse transcription step followed by the amplification of the resulting cDNA using PCR.
Polymerase Chain Reaction (PCR) is a well-established technique that allows the amplification of a specific DNA segment multiple times, making it easier to study and analyze. In PCRD, after the generation of cDNA through reverse transcription, the cDNA is amplified using PCR. This involves multiple cycles of alternating heating and cooling, during which DNA polymerase enzyme replicates the cDNA, resulting in an exponential increase in the number of specific DNA fragments.
Overall, PCRD enables studying the expression of specific genes, as it allows the amplification and detection of RNA molecules. It provides valuable insights into the transcriptional activity of genes and is widely used in research areas such as gene expression analysis, identification of gene variants, and studying diseases at the molecular level.
Common Misspellings for PCRD
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