NATIVEPAGE Meaning and
Definition
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NativePAGE is a technique used in molecular biology to separate and analyze proteins based on their native structure and charge. It is a commonly used method in protein purification, analysis, and characterization. The term "Native" refers to the preservation of the native conformation of proteins, meaning that the proteins are not denatured or unfolded during the process.
NativePAGE involves running proteins through a polyacrylamide gel matrix that contains a pH gradient, enabling the separation of proteins based on their charge. The pH gradient is established using a buffer system that generates a range of pH values within the gel matrix. Proteins, in their native state, migrate to a specific position in the gel based on their isoelectric point (pI), which is the pH at which they have a net charge of zero. This migration pattern allows for the separation of proteins by their charge and size.
The technique is particularly useful for studying membrane proteins, protein complexes, and proteins with post-translational modifications, as it preserves their native structure. NativePAGE can be used to analyze protein interactions, determine protein purity, assess protein aggregation, and study enzyme activity.
Overall, NativePAGE is a powerful tool in the field of molecular biology, allowing scientists to study proteins in their native form, providing valuable insights into their structure and function.
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