MOLISCH TEST Meaning and
Definition
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The Molisch test is a chemical assay used to detect the presence of carbohydrates in a given substance or solution. It was developed by the Austrian scientist Hans Molisch in the early 20th century. This test is primarily used in fields such as biochemistry, food science, and pharmaceutical research.
To perform the Molisch test, a few drops of a reagent known as Molisch reagent, which typically consists of alpha-naphthol dissolved in ethanol, are added to the sample being tested. The mixture is then heated and concentrated sulfuric acid is carefully added to it. If the substance being tested contains carbohydrates, a purple or violet ring will form at the interface of the two liquids. This is due to the formation of a complex between the carbohydrates and the components of the test, which causes a characteristic color change.
The Molisch test is based on the reaction between carbohydrates and the alpha-naphthol present in the reagent. Carbohydrates, such as glucose and fructose, react with the alpha-naphthol in the presence of sulfuric acid to form furfural compounds. These furfural compounds then condense with the alpha-naphthol to produce a colored product.
The Molisch test is commonly used as a preliminary screening test for the presence of carbohydrates in various samples, including food products, beverages, and biological fluids. It is a simple and sensitive assay that can detect even small amounts of carbohydrates. However, it should be noted that the test is not specific for carbohydrates and can also give a positive result for other compounds, such as phenols and aldehydes, which can also condense with the alpha-naphthol.
Common Misspellings for MOLISCH TEST
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Etymology of MOLISCH TEST
The word "Molisch Test" is named after the Austrian botanist and chemist Hans Molisch (1856-1937), who developed this test. The test is a biochemical method used to detect the presence of carbohydrates in a solution. Hans Molisch made significant contributions to the field of plant physiology and biochemistry and developed several tests and techniques that are still used today.
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