MLEI ENDONUCLEASE Meaning and
Definition
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MleI Endonuclease is a specific enzyme belonging to the class II type IIS restriction endonuclease family. It is derived from the bacterium Moraxella lwoffi and primarily functions to cleave DNA at specific recognition sites. This enzyme recognizes a unique sequence of nucleotides on the DNA molecule and catalyzes the hydrolysis of the phosphodiester bonds within that sequence.
The cleavage site recognized by MleI is asymmetric, located away from the center of the recognition sequence. Specifically, it recognizes the sequence 5'-TAAAK-3' (where A denotes adenine, K denotes thymine or guanine, and the cleavage occurs between the first A and the following base). This specific recognition and cleavage pattern allows MleI to generate blunt ends, leaving no overhanging nucleotides.
Like other endonucleases, MleI plays a critical role in molecular biology and genetic engineering applications. It is frequently used in recombinant DNA technology to precisely manipulate DNA molecules, including cutting and joining specific DNA fragments. MleI is commonly employed for DNA cloning, gene expression analysis, genotyping, and other molecular biology techniques that require site-specific DNA cleavage.
The use of MleI Endonuclease offers researchers greater control and versatility in designing and engineering DNA molecules for various genetic studies. Its specificity, reliability, and ability to generate blunt ends make it a valuable tool in the field of molecular biology.
Common Misspellings for MLEI ENDONUCLEASE
- nlei endonuclease
- klei endonuclease
- jlei endonuclease
- mkei endonuclease
- mpei endonuclease
- moei endonuclease
- mlwi endonuclease
- mlsi endonuclease
- mldi endonuclease
- mlri endonuclease
- ml4i endonuclease
- ml3i endonuclease
- mleu endonuclease
- mlej endonuclease
- mlek endonuclease
- mleo endonuclease
- mle9 endonuclease
- mle8 endonuclease
- mlei wndonuclease
- mlei sndonuclease
Etymology of MLEI ENDONUCLEASE
The word "MleI" refers to a type II restriction endonuclease enzyme isolated from the bacterium Moraxella lwoffi. The etymology of the term "MleI" is based on the naming conventions for restriction enzymes, which generally derive from the source organism's name or some other characteristic.
In this case, "Mle" refers to the first three letters of the genus name Moraxella, while the letter "I" indicates that MleI is the first enzyme isolated from this bacterium. The designation "endonuclease" is added to indicate that MleI is an enzyme that cleaves the DNA molecule at specific internal locations.
Overall, the etymology of "MleI Endonuclease" reflects its origin from Moraxella lwoffi, as well as its classification as an endonuclease.
